Light-sheet fluorescence microscopy is a state-of-the art imaging technique for live-cell imaging. Unlike traditional imaging techniques such as laser scanning and spinning disk confocal microscopy ...

Lattice light sheet microscopy is a newer light sheet microscopy technique that is primarily used in biological settings. It is a method that uses sheets of light in a lattice conformation to take ...

Optimal image quality in light-sheet microscopy requires a perfect overlap between the illuminating light sheet and the focal plane of the detection objective. However, mismatches between the ...

The starting point for the design of light-sheet systems should be a specific biological question or application. Indeed, LSFM is particularly suited for construction around the sample, because the ...

A research group led by Takashi Saito, of the Ehime University Graduate School of Medicine, developed a 2-photon excitation light-sheet fluorescence microscope which (1) lowers phototoxicity, (2) ...

Tools for optical imaging of brain activity in freely moving animals have considerable potential for expanding the scientific understanding of the brain. However, existing technologies for imaging ...

Since 2004, when the first paper on light-sheet microscopy (aka selective plane illumination microscopy, or SPIM) appeared (Science, 305:1007-09), users of the technique have coalesced into a growing ...

Light microscopy is a cornerstone in life sciences technology, having experienced considerable diversification since its inception. This development becomes visible when considering the distinctive ...

Tools that allow neuroscientists to record and quantify functional activity within the living brain are in great demand. Traditionally, researchers have used techniques such as functional magnetic ...

A video showing the birth of an incredible network of neurons in the nervous system of a Zebrafish has won first prize at the Nikon Small World in Motion Awards, which highlight the best in ...