Nov 10, 2025 · A complete guide to calculating, visualizing, and standardizing qPCR data for rigorous, reproducible scientific publication.

For analysis: almost all qPCR data will fall on a log-normal distribution, so you should usually be doing stats on log data, accordingly. Using dCt values directly is easiest (though remember...

In this section, general guidelines and a sample gene expression experiment are presented to demonstrate how to perform gene expression analysis using real-time PCR. A sample gene …

Aug 21, 2025 · Effectively present your qPCR data. Learn strategies for accurate visualization, robust analysis, and clear communication of complex scientific findings.

The internet is filled with different methods, procedures, and workflows that can overwhelm you during data analysis, especially if this is your first time with RT-qPCR. This article is going to …

Feb 27, 2014 · How do I publish qPCR data in a bar graph? I have my raw data according to two groups of different types of animals that are being tested with pharmacological compounds or …

Don't use ddCt values, because this is a very 'lossy' format: you take data where you can present individual sample expression values and make it instantly clear to the reader how consistently...

How to present data for qPCR? I ran qPCR to study the expression of genes in my samples but they were not treated. It was just to see the expression at a certain point of time.

Jun 17, 2023 · Using these steps you can conduct your qPCR analysis wherever you are, even if you’re on a road trip. To make things even easier, you can create an Excel template to use …

In this document we describe some of the crucial steps in qPCR data analysis and illustrate statistical notions with a concrete example using the RealTime StatMiner software.